Guide-it SNP Screening Kit oligo design tool
Design tool guidelines:
- Input sequences must include exactly one SNP
- Input sequences must be the same length and should include at least 35 bases on either side of the SNP site
(for optimal probe design we recommend including ≥50 bases on either side)
- Input sequences must not exceed 1,000 bases in length
- Please be careful to avoid the inclusion of extra spaces when inputting sequences, as these will be interpreted as part of the input and will likely return an error message
- Following the submission of sequences, users can return to the input screen by clicking the "Back" button of the web browser
Example:
Sequence before editing: GTCCGAGAACACCTTTATTGTGCACGTCCCCCGCAGAGCAGCCTCAGGCGTCCTGGTAGTAGTTGTTGAAGTTGATGCGCAGCAGAAAGT
Sequence after editing: GTCCGAGAACACCTTTATTGTGCACGTCCCCCGCAGAGCAGCCTCCGGCGTCCTGGTAGTAGTTGTTGAAGTTGATGCGCAGCAGAAAGT